MCID Core Software


MCID™ is a powerful imaging platform, incorporating many years of development, MCID™ is in use at hundreds of the world’s most top research laboratories. 

In addition to functions for image editing, annotation, enhancement and archiving, MCID™ offers fully configured functions for fluorescence microscopy, automated image stitching, quantitative and whole-body autoradiography, gel/blot analysis, grain & cell counting and stereology. The system supports a broad variety of CCD cameras, automated microscope stages, shutters, filter wheels and other peripherals. An object orientated macro editor simplifies operation in repetitive tasks.

MCID™ is ideal for a core imaging facility. It is a simple matter to create a distributed image analysis environment in which you can analyse images at any computer. In this unified imaging environment, the software tools need only be learned once, and exchange of images and data between systems is completely transparent.

Quantitative Regional Autoradiography

Functions include precise calibration to radiolabel standards, with curve fitting, extrapolation (improves preci-sion of calibration extremes) and algorithms that make data comparable across detection technologies (e.g. film and phosphor plate). Use for regional receptor binding and functional mapping. Operational equations for cerebral glucose utilization, protein synthesis rates, and blood flow are included.

Grain, Cells and Particle Counts Analysis

This generic counting mode is applicable to cells, grains and other discrete objects. Valid targets are dis-criminated from background using intensity, color and spatial criteria. Image transformation and combina-tion functions assist in discriminating difficult targets (e.g. particulates overlapped with cells).

Whole Body Autoradiography Analysis

Accepts digital images from any phosphor imager, or film images from cameras and scanners. Automated sampling tools simplify data gathering. Excellent support for very large image files.

Gel Analysis

Acquire, annotate, archive and print using almost any gel scanner or CCD based acquisition system. Ana-lyse lanes or thin layer chromatographs. Includes linear and nonlinear calibration in two dimensions (e.g. to molecular weight and pH), corrections for gel artifacts, automated and manual lane and peak detection, and flexible baseline subtraction.


here is support for monochrome and color integrating cameras, and for ultra-low light cameras. Extensive image fusion functions (including flexible adjustment of color, intensity, and transparency of each discrete fluorochrome) combine images of different fluorochromes. Digital confocal functions deconvolve using near-est-neighbour or no-neighbor algorithms. Image-stitch fluorescence images. Use any of the MCID measure-ment functions to quantify fluorescence signal.

Intuitive Image Analysis & Capture

MCID Analysis has been designed is an image analysis software solution for offline analysis of images. MCID Analysis is ideal for of images where image capture is not required. MCID Analysis includes all the powerful analysis tools of MCID but without the hardware and capture support. MCID Analysis is an ideal solution for MPhosphor Imagers and other capture systems.

Feature Summary


MCID is a modular applications based acquisition and image analysis software. MCID Core is easily optimized and configured for single applications based on specific user requirements. MCID uses a single monitor, and displays the aquired image and the analysis data in the same window. Therefore, we recommend a high resolution display, typically 1280 x 1024 pixels x 24 bits (16 million colors). Larger monitors at 1600 x 1200 pixels are also available. MCID can also support dual view technology via a suitable graphics card from NVIDIA.

Independant Image Channels

MCID offers multiple, independent image channels. Each channel can contain a discrete, calibrated image. Use the multiple image channels to align stained sections and autoradiographs, or to display images acquired at different fluorescence wavelengths.

Image Input / Saving

MCID accepts and exports images in a variety of formats, including monochrome and color TIFF, MCID, and in the native formats of many cameras and scanners. For other file formats, a general-purpose image format editor is supplied as standard. You can easily create your own image file format specifications, and integrate them within MCID.


Calibrate images to external density and spatial standards. Nonlinear calibrations (e.g. molecular weight) fully supported.

Image Fusion

Collect any number of discrete fluor images. Fusion functions create 24-bit color images. Functions range from simple addition to weighted addition with layer-specific transparency.

Image Processing

Apply filters, logical operators, image arithmetic. Edit images to remove artefacts, or to create image montages.

Easy Report Generation and Data Export to excel

Report generator creates summary reports including numerical data, profile graphs, and image. Print reports with one command.

Gel / Blot Analysis

Nonlinear calibrations in X and Y dimensions. 1D mode. 1D mode for TLC. Corrections for bent or stretched lanes, smiling. Vertical, horizontal, rotatable, and user-traced lane definition tools. Display and analyze multiple lanes, simultaneously, or one at a time. Manual or automatic peak detection. Manual or automatic baseline correction.

Powerful Profile Tools

Vertical, horizontal, rotatable, and user-traced lane definition tools. Display and analyze multiple profiles, simultaneously, or one at a time. Zoom in on any portion of the profile for high-resolution display.

Image Sampling Tools

Sampling tools are probes used to gather data from images. Tools can be sized and rotated by the user.

Powerful yet simple to use

Rectangle, Template, Distance, Circle, Paint, Angle, Trace, Polygon, Pixel Report and Autoscan Sample Tools

Target Detection

For grain counts, and other feature distribution analyses, fully automated target detection is supported. Multiple density thresholds can be combined with logical criteria (e.g. area) to separate targets from background.

Everything you need in one solution

mcid core image capture and analysis

Digital Densitometry System

The MCID Densitometry System has served thousands of scientists for more than 20 years. The system includes calibrations and corrections which are easily applied to yield precise quantitative and semiquantitative data. The MCID Densitometry System can be used with a wide variety of cameras, scanners and phosphor images.

Whats included

MCID Software
Retiga R1 USB Camera
Macro Accessory Package (lightbox, Camera Stand, lense and calibration reticule)
Optional Dell PC (Precision Workstation with one or two monitors)


  • Calibrate to any concentration or density standards
  • Automatic data optimization functions include thresholding and variance based pixel selection
  • Operational Equations convert optical density values to rates of local cerebral glucose utilization, protein synthesis, or blood flow
  • Real time alignment of autorad with NSB or stained section
  • Automated sampling tools
    Multiple image operations


  • Fully quantitative analysis
  • Advanced measurement of clipped and saturated pixels for improved data quality
  • A complete solution for densitometry
  • Easy redirected sampling and subtraction of NSB
  • Use one software package for all your densitometry needs
  • Fast and easy
  • Simple application to multilabel studies
mcid core image capture and analysis

Gel & Blot Analysis

MCID acquires, annotates, archives, prints and quantifies images of your gels and blots. Use MCID as a simple archival system or take advantage of the extensive list of analysis functions. Digitize your specimens with any input device, including scanners, and video or digital cameras.

Whats included

MCID Software
Retiga R1 USB Camera
Macro Accessory Package (lightbox, Camera Stand, lense and calibration reticule)
Optional Dell PC (Precision Workstation with one or two monitors)


  • Analyse radiolabelled, stained, luminescent and fluorescent gels and blots
  • Includes linear and non-linear calibrations
  • Apply corrections for gel artifacts
  • Automated and manual lane and peak detection
  • Automated baseline subtraction
  • Full annotating, viewing and editing functions


  • Sample versatility
  • Calibrate to molecular weight and pH
  • Improved data quality
  • East to use
  • Simple background corrections for improved data quality
  • All your gel documentation needs in one package

How can MCID help you?

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